![]() As control, aggregation parameters of erythrocytes from the same donor were used. Each series of experiments with given concentrations of fibrinogen was performed on blood from one donor. The final concentration of fibrinogen was 5 to 9 g/l in different series of experiments. When studying the effect of fibrinogen concentration on the aggregation of erythrocytes, dry fibrinogen (Bio Chemika) was added to their suspension. Degree of erythrocyte disaggregation in percentage of Ma at 10 sec -1, 15 sec -1, 20 sec -1 sheer rate - D 10, D 15, D 20.ĭuring the study of the influence of plasma factors on erythrocytes aggregation the main attention was paid to concentration of fibrinogen, products of its transformation - fibrin monomers and oxidized fibrinogen and also to the possibility of blocking fibrinogen receptors of erythrocytes membranes by monoclonal antibodies of fibrinogen receptors of platelets. Amplitude of aggregatogram at 40 sec after the start of the aggregation process (mm) - A 40. Degree of aggregation according to the maximum amplitude of aggregatogram (mm) - Ma. Erythrocyte aggregation and disaggregation were assessed using the following parameters:ġ. The process of erythrocyte disaggregation was recorded when creating sheer stress, set at the exact rate. The process of aggregation was recorded during hydrodynamic mixing and upon its stop. Plasma was separated from erythrocyte mass, leukocytes and platelets removed, and after that plasma and erythrocyte mass were mixed in 2:1 ratio respectively.Įrythrocyte aggregation was studied by a rheoscope constructed using the method of H.Schmid-Schönbein et al. ![]() The blood was stabilized with 3.8% sodium citrate solution in 9:1 ratio and centrifuged for 20 min at 3000 rpm. The study was performed on 48 blood samples from patients in the acute period of burn disease (a second- or third-degree burn >20% of total body surface area) and 81 blood samples from healthy volunteers. It is important to research these issues as it determines the therapy tactics aimed at correction of microcirculatory disorders in thermal trauma. It has not even been determined which factors, plasma or cellular, cause the disorders of red blood cell aggregation in burn disease, or whether they are reversible. The mechanism of blood cell hyperaggregation in burn disease is still unclear. The increase in erythrocyte aggregation may be connected with the influence of plasma factors (increase in proteolysis, free radical oxidation, concentration of high molecular weight proteins, etc.) as well as with cellular factors (transmembrane redistribution of phospholipids, change of neuraminic acid content in membranes, change of level of endocellular Ca 2+, etc). First of all, it’s refers to erythrocyte aggregation, as its increase in combination with decrease in disaggregation causes the development of the syndrome of hyper viscosity of blood. Vasoconstriction passes soon and other factors, mainly hemorheological, determine microcirculatory disorders. Generalized vasoconstriction of adducting micro vessels is the first, though a very unstable response to the trauma. Reduction of oxygen service of vitals is always revealed after a major burn injury. Microcirculatory disorders always occur after thermal trauma and determine the development of such complications as acute renal insufficiency, respiratory distress, multiple organ dysfunction syndrome. Based on these results the authors conclude that in burn patients erythrocytes aggregation is affected by changes in the contents of blood plasma, specifically fibrinogen and the product of its transformation - fibrin fibrin monomer. This increase was accompanied by a dose-dependent increase in erythrocytes aggregation. The level of medium molecular peptides increased sharply in blood plasma from burn patients. ![]() However, correlation between the level of oxidized fibrinogen and erythrocytes aggregation was not found. The level of oxidized fibrinogen in blood plasma of burn patients increased by about two fold. The aggregation was not affected by monoclonal antibodies against platelet GPIIb/IIIa receptors. It is found that fibrinogen-induced aggregation of erythrocytes is accompanied by “saturation” effect. ![]() The rate and degree of aggregation was also increased as the fibrinogen concentration increased. We show that normalization of blood plasma contents completely restores erythrocytes aggregation and disaggregation of burn patients. Results showed that the rate and degree of erythrocytes aggregation increased significantly in burn patients, and what is especially unfavorable for microcirculation, erythrocytes disaggregation decreased. The manuscript describes experiments designed to examine factors that influence erythrocytes aggregation within the blood of burn patients.
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